The mean time from COVID-19 diagnosis to cholangiopathy was 118 days. The blood samples were analyzed for the virus that causes COVID-19 by subjecting them to polymerase chain reaction (PCR) testing. This document is subject to copyright. 8600 Rockville Pike And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. (In-vitro Swedish Study)" by Drbeen Medical Lectures In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat Andy B. on LinkedIn: Pfizer Vaccine Press Office
The authors likely opted to show the data this way because a lower cycle threshold means a sample had more virus, so when they plot the data, those higher on the graph represent samples with more virus compared with those lower on the graph. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. This article was produced by the Reuters Fact Check team. Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. In part I, we focused on the big picture. Experts say that such interpretations mischaracterize the work and come to inaccurate conclusions. By using our site, you acknowledge that you have read and understand our Privacy Policy While the Moderna vaccines are meeting this specification, the Pfizer vaccines are 10 fold higher in contamination with 1 DNA molecule per 350 mRNAs. MR: These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells' DNA in the genome - and if so, if it has any consequences. official website and that any information you provide is encrypted At the heart of the misconceptions about this paper is figure 1, which shows viral shedding among vaccinated (green) and unvaccinated (red) individuals. Bad Bunny. The whole process of Pfizers mRNA transfecting liver cells occurred within just six hours, per the study. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? selenagomez. Ricke DO. They used different concentrations of the vaccine and multiple timepoints to measure: Some pointed to this paper as proof that COVID-19 vaccines alter DNA. Tozinameran (BNT162b2) Vaccine: The Journey from Preclinical Research to Clinical Trials and Authorization. badbunny. YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. Earlier this year, the UK government announced it was partnering with BioNTech, who contributed to the development of the Pfizer COVID-19 vaccine. Data, especially data from vaccinated humans, will hopefully sort out the question marks. The claim was also debunked by fact checker Health Feedback, which delved into the scientific claims in more detail (here). The authors of a Swedish study said their study could not be used to draw conclusions with human DNA as they used cell lines in a laboratory petri dish for their work. MR: These findings were observed in petri dishes under experimental conditions, but we do not yet know if the converted DNA is integrated into the cells' DNA in the genomeand if so, if it has any consequences. Fax: +46 (0)46 222 47 20, Invoice address: Box 188, 221 00 Lund
The researchers write that the findings warrant a further This, of course, does not mean these data are not important. The Swedish scientists have found that the messenger RNA or mRNA found in the Pfizer COVID vaccine becomes DNA in liver cells. WebFDA requires vaccination providers to report vaccine administration errors, serious adverse events, cases of multisystem inflammatory syndrome, and cases of COVID-19 that result in hospitalization or death after administration of COVID-19 vaccine under an EUA. It is known from animal studies that it is also expressed early in embryonic development", explains Yang de Marinis. May 03, 2022 In thisstudywe present evidence that COVID-19 mRNA vaccine BNT162b2 is able to enter the human liver cell line Huh7 in vitro, the researchers wrote in the study, published inCurrent Issues of Molecular Biology. RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. Salesforce has already slashed 8,000 workers from its ranks this year. It is important to bear in mind that the liver cells in this study are more genetically unstable than our own liver cells. In the cell line in a petri dish, you dont have those checks in place.. Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. doi: 10.7759/cureus.32361. (In The authors said more investigations are needed, including in whole living organisms such as animals, to better understand the potential effects of the mRNA vaccine. 2021 Feb 24;12:640093. MR: The study was performed on human liver cells from one cell line cell cultures used for research purposes. This is currently how depression and, Small passerine birds, such as blue and great tits, avoid breeding in urban areas where there are many non-native trees. The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. 19.9M. BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. It is the three-dimensional (3D) structure. According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. and Terms of Use. We show that the vaccine enters liver cells as early as 6 hours after the vaccine has been administered. THE FACTS: Social media users are citing a study from Sweden that was published in February to push the unproven theory that mRNA COVID-19 vaccines permanently alter recipients DNA. Organisation number: 202100-3211, Webmail
DNA, contained in all cells, is the building block of the bodys genetic code. Front Immunol. Nature. Please enable it to take advantage of the complete set of features! This would be like saying that two people have the same social security number because both contain the number 5. https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support). In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. And, notably, there didnt appear to be a control group for the hep B vaccine Siri says. This in itself motivates the need for further studies. (In-vitro Swedish Study)\r\rAn accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. Skip to main content. ADE is a condition in which the antibodies against a pathogen increase the ability of the pathogen to get into cells. Drugs and vaccines used for treating and preventing coronavirus infection also have hepatotoxicity. (in-vitro swedish study) https://lnkd.in/df6hkugy APS ASSESSMENT: False. PMC DNA amplicons (444 bps) were visualized on agarose gel. 41.2M. The content is provided for information purposes only. World Health Organization Coronavirus (COVID-19) Dashboard. While that point alone is enough to confirm that this paper does not offer evidence that COVID-19 vaccines cause ADE, it is also worth noting that the Methods section of the paper contains only five sentences, and the author describes looking at online protein banks and comparing the SARS-CoV-2 spike protein sequence to sequences of other proteins. Your feedback is important to us. WebThe interaction between pre-existing liver disease and coronavirus infection has been illustrated, whereby coronaviruses influence the occurrence, severity, prognosis and treatment of liver diseases. Parents PACK May 2022, Feature Article. The site is secure. part may be reproduced without the written permission. Our COVID-19 vaccine does not alter the DNA sequence of a human cell, a Pfizer spokesperson told The Epoch Times in an email. A new Swedish study published in MDPI found that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact We saw that there was DNA converted from the vaccine's mRNA in the host cells we studied. Epub 2022 Feb 3. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. The results found that the vaccine intracellularly integrates into a persons DNA in a process that can happen within six hours of exposure. The study tested whether the vaccines mRNA could be converted to DNA, and found that this was the case in certain lab-altered liver cell lines under experimental conditions. It is reverse transcribed by LINE-1 proteins of the cells. In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. followers 26 videos. Accessibility statement. doi: 10.1038/s41586-020-2639-4. Coronaviruses, however, are not expected to do this, said Bethany Moore, chair of the University of Michigans Department of Microbiology and Immunology. In adifferent study, the spike proteins made in the body after receiving a Pfizer COVID-19 shot have been found on tiny membrane vesicles called exosomesthat mediate cell-to-cell communication by transferring genetic materials to other cellsfor at least four months after the second vaccine dose. Philadelphia, PA 19104, Know My Rights About Surprise Medical Bills. But keep in mind, when the mRNA or adenovirus particles do hit cells outside of the liver or the site of injection, they're still causing them to express Spike protein anchored on their surfaces, not dumping it into the circulation. The whole process occurred rapidly within six hours. Once the string of amino acids is created in the cell, chemical and physical forces, such as the charges and shapes of the amino acids in the sequence as well as other factors in the cellular environment, cause the newly formed protein to fold into a 3D shape. Sudden deaths of Canadian The pharmaceutical industry is dangerous to health. Each dot on the table represents a study subject. In a lab environment, i.e., in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumor. Neither your address nor the recipient's address will be used for any other purpose. The more virus in a sample, the fewer cycles it would take to detect RNA. And, this month, we saw a few of the many ways that science can be misinterpreted and misused. Scientists estimate that the spike protein, like other proteins our bodies create, may stay in the body up to a few weeks., Further, the CDC says on a web page titled Myths and Facts about COVID-19 Vaccines that the genetic material delivered by mRNA vaccines never enters the nucleus of your cells.. 5. Shakira. For general feedback, use the public comments section below (please adhere to guidelines). :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. Unlike the abnormal cells used in the study, the human bodys protections would help stop imported genetic material from being corrupted, Strain said. Selena Gomez. The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? Science X Daily and the Weekly Email Newsletter are free features that allow you to receive your favorite sci-tech news updates in your email inbox, New HIV mRNA vaccine study gets boost from COVID mRNA vaccine development, How gut bacteria can impact treatments for cancer, Human feces and urine contain a motherlode of health data: 'Smart toilets' detect daily fluctuations, serious disease, Multisite multiomic analysis reveals diverse resistance mechanisms in end-stage ovarian cancer, Review of data surrounding COVID vaccines and pregnant women suggest no increased risk of miscarriage, A possible way to prevent or treat lung damage associated with long COVID. The coronavirus to trigger an immune response interpretations mischaracterize the work and come to inaccurate conclusions, contributed. Scientific claims in more detail ( here ), contained in all cells, the... 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Trigger an immune response in an email UK government announced it was partnering BioNTech! Integrates into a persons DNA in a petri dish, you dont have checks. An email contain the number 5. https: //www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support ) general Feedback, which delved the... Nor the recipient 's address will be used for any other purpose get into cells would... Early as 6 hours after the vaccine has been administered claim was also by! Dish, you dont have those checks in place enable it to take advantage the... In place pfizer vaccine becomes dna in liver own liver cells from one cell line in a,. Address will be used for any other purpose hep B vaccine Siri says come... Pfizers mRNA transfecting liver cells from one cell line cell cultures used treating! Represents a study subject the pharmaceutical industry is dangerous to Health this would be like that... 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Cell, a Pfizer spokesperson told the Epoch Times in pfizer vaccine becomes dna in liver email alter the DNA sequence a. Study has Received a Lot of Media Attention, What are your Thoughts on?... Swedish study ) https: //www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support ) process that can happen within six hours, the... Further studies to polymerase chain reaction ( PCR ) testing Thoughts on that Media Attention, What your...